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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
09/11/2017 |
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Actualizado : |
12/12/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Soil and rhizosphere bacterial diversity in maize agro-ecosystem. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 35-51. |
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ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
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DOI : |
10.5539/sar.v6n3p |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. |
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Contenido : |
ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial ... Presentar Todo |
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Palabras claves : |
DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668
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Marc : |
LEADER 02541naa a2200289 a 4500
001 1057747
005 2018-12-12
008 2017 bl uuuu u00u1 u #d
022 $a1927-050X (Print) // 1927-0518 (Online)
024 7 $a10.5539/sar.v6n3p$2DOI
100 1 $aFEDERICI, M.
245 $aSoil and rhizosphere bacterial diversity in maize agro-ecosystem.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017.
520 $aABSTRACT. Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices. Copyright © Canadian Center of Science and Education
653 $aDGGE
653 $aMETAGENOMICS
653 $aPYROSEQUENCING
653 $aRHIZOSPHERE
653 $aSOIL BACTERIA
653 $aTRANSGENIC MAIZE
700 1 $aBAJSA, N.
700 1 $aLAGURARA, P.
700 1 $aREVALE, S.
700 1 $aMARCONDES, J.A.
700 1 $aDALLA RIZZA, M.
773 $tSustainable Agriculture Research, 2017$gv.6 (3): 35-51.
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INIA Las Brujas (LB) |
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| 1. |  | DELGADO, S.; ALLIAUME, F.; GARCÍA PRÉCHAC, F.; HERNÁNDEZ, J. Efecto de las plantaciones de Eucalyptus sp. sobre el recurso suelo en Uruguay. (The effects of Eucalyptus sp plantations on Uruguay natural resources. Part II: Soils). Agrociencia Uruguay, 2006, v. 10, no. 2, p. 95-107. Article history: Recibido: 06/03/06; Aceptado: 25/10/06. AGRADECIMIENTOS: A todas las fuentes de financiación de los proyectos desarrollados y en marcha, Dirección Forestal del MGAP, Banco Mundial, Comisión Sectorial de Investigación...| Biblioteca(s): INIA Tacuarembó. |
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| 2. | | CALVO, M.C.; DELGADO, S.; DUARTE, F.; GARCIA, F.; SCATONI, I.; GONZÁLEZ, A. Fruit flies (Diptera: Tephritidae) and hosts association in different ecosystems in Uruguay. International Journal of Pest Management, 2022, vol. 68, issue 4: "Uruguayan Society of Phytopathology (SUFIT): Plant protection for a sustainable agriculture", p.369-380. doi: https://doi.org/10.1080/09670874.2022.2126023 Article history: Received 30 April 2022, Accepted 10 September 2022, Published online: 11 November 2022. Funding: The authors want to thank Emily Silva and Mity Schlesener from UFPel and Eugenia Lorenzo from the UDELAR for their support,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 4. | | DUARTE, F.; CARO, A.; DELGADO, S.; ASFENNATO, A.; LÓPEZ, L.; HERNÁNDEZ, F.; CALVO, M. Sterile insect technique (sit) effectiveness to control Ceratitis capitata (Diptera: Tephritidae) and medfly catches in two mass trapping layouts. International Journal of Pest Management, 2022, vol. 68, issue 4: "Uruguayan Society of Phytopathology (SUFIT): Plant protection for a sustainable agriculture", p.402-413. doi: https://doi.org/10.1080/09670874.2022.2130468 Article history: Received 06 May 2022, Accepted 21 September 2022, Published online: 11 November 2022. Funding:
This work was supported by the National Agency for Research and Innovation-ANII; the company ENTAISLE S.A. and the company...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 5. | | SILVEIRA, L.; CHRETIES, CH.; ALONSO, J.; AMORÍN, C.; DE IZAGUIRRE, P.; CRISCI, M.; SYMONDS, S.; MARTÍNEZ, L.; GARCÍA, M.; GARCÍA, F.; DELGADO, S.; CLÉRICI, C.; BENTANCOR, L.; HILL, M.; ALLIAUME, F.; CABRAL, P.; AUDICIO, P.; IROUMÉ, A.; HUBER, A.; SCHIPILOV, A. Efectos de la actividad forestal sobre los recursos suelos y aguas Montevideo (UY): INIA, 2011. 41 p. (Serie FPTA-INIA; 32) "Proyecto FPTA 210: "Efecto de la actividad forestal sobre los recursos suelos y aguas, en microcuencas similares sometidas a distinto manejo". Período de Ejecución: Mar. 2007-Jun. 2010| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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